OsteoPerio Academy / Illustrated chapter 01
Mucogingival grafting.
Understand the design.
CAF, tunnel and VISTA: follow the anatomy, the graft and the forces that hold the tissues in position.
A literature-based chapter for qualified dentists and postgraduate learners. Access design, graft choice and suturing are three connected decisions—not one interchangeable technique.
Explore the chapter- Cemento-enamel junction (CEJ): the anatomical reference.
- Exposed root: assess depth, width and surface condition.
- Papilla: assess interdental attachment clinically.
- Mucogingival junction: evaluate the available tissue.
01 / Assessment
Read the defect before choosing the access.
Establish the patient’s concern, plaque control and periodontal stability. Record recession dimensions, CEJ detectability, cervical lesions, phenotype, keratinized tissue, vestibular anatomy and tooth position. Recession alone does not automatically require surgery.[1][2]
RT1
No interproximal attachment loss.
RT2
Interproximal attachment loss is less than or equal to buccal attachment loss.
RT3
Interproximal attachment loss is greater than buccal attachment loss.
Cairo classification uses attachment measurements, not the apparent papilla height in a photograph. It helps frame coverage potential; it does not promise complete coverage or prescribe CAF, tunnel or VISTA.[1]
02 / Graft and recipient design
Separate access design from graft choice.
CAF, tunnel and VISTA describe access and tissue positioning. A connective-tissue graft describes an added tissue layer. Choose the biological objective and the recipient design separately, then make them work together.[3][5][7]
CTG: connective-tissue graft
Commonly placed under a covering flap or tunnel for root coverage and / or phenotype modification. The gold layer represents connective tissue, not a membrane or bone graft.
- Covering soft tissue.
- Connective-tissue graft.
- Vascular recipient bed; its preparation depends on the site.
FGG: free gingival graft
An epithelialized graft often used to augment keratinized tissue. Its exposed surface, appearance and treatment objective differ from a buried CTG; it is not simply another name for CTG.
- Exposed epithelial surface over the graft.
- Prepared vascular recipient bed.
Autogenous grafting adds a donor-site wound. Substitutes may avoid harvesting, but their performance depends on the material and indication; they are not automatically equivalent to CTG. These figures explain layer relationships, not harvesting dimensions or a universal graft prescription.[3][5]
| Access family | Entry | Papillary continuity | Preparation plane |
|---|---|---|---|
| CAF | Marginal / papillary design; releasing incisions in some variants. | Papillary preparation follows the selected flap design. | Thickness and release vary with the design and site. |
| Tunnel | Sulcular access into connected recipient spaces. | Papillary bridge retained. | Partial, full or mixed planes, depending on the described variant. |
| Original VISTA | Remote vestibular incision. | Marginal tissues and papillae kept continuous. | Subperiosteal access in the original description. |
Confirm the exact operative variant in its source before applying these distinctions.[4][7][12][13]
03 / Coronally advanced flap
Move the flap. Support the graft.
CAF mobilizes the local soft tissue and advances its margin coronally. A CTG can be added underneath. The illustration uses an envelope concept for adjacent recessions; CAF also includes designs with releasing incisions.[4][5]
Concept 1
Define the access
Plan the marginal and papillary design around the defect and the available tissue. Preserve an appropriate vascular supply.
- Marginal / papillary access in an envelope design.
- Interdental tissue: its dimensions and preparation matter.
Concept 2
Create passive mobility
Release the flap sufficiently for passive coronal positioning. If indicated, adapt a CTG to the recipient bed before covering it.
- CTG positioned over the target roots and recipient bed.
- Covering flap shown transparent for teaching.
Concept 3
Cover and stabilize
Adapt the flap over the graft and secure its planned position. Sutures hold a mobile flap; they should not force an inadequately released flap into place.
- Tooth-supported stabilization, shown schematically.
- Planned margin position; not a healed result.
- When to consider it
- A flap can be mobilized predictably and the papillary / marginal anatomy supports the chosen design.
- What to question
- Flap thickness, available keratinized tissue, interdental support and tension influence planning. A releasing incision is not compulsory in every CAF.
- How to read the evidence
- A systematic review supports adding CTG to CAF for selected single recessions without interdental attachment loss. That evidence should not be extended to every defect or every flap variant.
Conceptual sequence, not a complete operative protocol. Planes, grafts and stabilization differ across published variants.[4][5]
OsteoPerio Academy / Illustrated procedure
One graft. Two sites. A coordinated sequence.
Watch a three-dimensional teaching film (3 min 4 sec) of an isolated-recession CAF with a de-epithelialized gingival graft (DGG). Follow recipient preparation, palatal incisions and harvesting, extraoral de-epithelialization, graft fixation and suspensory suturing.
English teaching draft · Clinical review pending · Silent, with written explanations · No autoplay
Use the video controls to pause, change speed or enter fullscreen. Selecting a chapter pauses the film at that step.
Step 1 of 12 / 0:00
Plan two connected sites
This film uses one isolated upper-tooth recession and a trapezoidal CAF with a de-epithelialized palatal graft. It differs from the multiple-recession envelope illustration above. Diagnosis, inflammation control, consent and anaesthesia precede the operative scenes.
Clinical check: The CEJ is the enamel–root landmark. The target position depends on the defect and cervical anatomy.
Read the complete sequence and scientific sources
An original educational synthesis of the isolated CAF design and DGG literature, rather than a reproduction of one paper’s complete protocol. Donor and recipient views alternate; layer spacing and instrument movements are simplified. Assessment, medical preparation, anatomical precautions and postoperative prescriptions require supervised clinical teaching.
1. Plan two connected sites (0:00)
This film uses one isolated upper-tooth recession and a trapezoidal CAF with a de-epithelialized palatal graft. It differs from the multiple-recession envelope illustration above. Diagnosis, inflammation control, consent and anaesthesia precede the operative scenes.
The CEJ is the enamel–root landmark. The target position depends on the defect and cervical anatomy.
2. Design the recipient access (0:10)
Marginal and papillary access is extended by two divergent releases into the mobile mucosa. This is one CAF variant; it does not mean that releasing incisions are needed for every recession.
Maintain a viable flap and appropriate papillary dimensions; the illustration gives no incision measurements.
3. Elevate and mobilize the flap (0:20)
The split–full–split concept preserves tissue for adaptation while allowing coronal movement. The three-dimensional view shows mobility; it does not reveal every dissection plane. Apical release is tailored to the anatomy.
The flap must reach the planned position passively. Sutures cannot substitute for adequate mobility.
4. Prepare the receiving surfaces (0:30)
The recipient is prepared and measured before graft harvesting in this example. Connective-tissue papillary beds accept the surgical papillae. Root-surface preparation is conservative and related to the actual surface findings.
No mandatory chemical conditioner or aggressive removal of root structure is implied.
5. Harvest the palatal graft (0:40)
Follow the coronal and apical horizontal incisions, the two connecting vertical incisions, then the split-thickness separation toward the palatal vault. A 2 mm collar is measured along the modeled surface in this example, following a later published protocol. The original 2010 study described 1–1.5 mm instead. The illustrated No.15 instrument is not a replica of a particular author’s instrument or a patient-specific cutting depth.
The blade changes from an upright outline cut to a near-parallel undercut. Preserve residual donor tissue; depth and vascular precautions are individual.
6. Remove the epithelium extraorally (1:12)
The graft is supported on moist sterile gauze while the connected scalpel blade follows a near-parallel plane to its surface. The animation shows superficial epithelial removal and the remaining connective tissue. A No.15 blade is illustrated; the cited extraoral protocols used a 15C. Dimensions and movements remain an educational model.
The resulting DGG is used as connective tissue beneath the CAF, rather than left as an epithelialized onlay.
7. Protect the palatal donor bed (1:32)
Unlike a single-incision subepithelial harvest, this surface wound is not shown primarily closed. A protective haemostatic dressing and its retention are illustrated as one approach; subsequent healing and review need individual care.
Blue lines retain the dressing. They do not pull the epithelial wound edges together over the harvested area.
8. Adapt the connective tissue (1:42)
The graft is brought to the prepared site, oriented and adapted without folds. Its relationship to the root, the adjacent connective tissue and the planned covering flap matters more than a fixed graft dimension.
A root surface itself is avascular; contact with the surrounding vascular tissues supports graft survival.
9. Stabilize the graft (1:52)
Peripheral graft fixation is shown schematically. The choice of anchorage and material follows the actual bed and flap design. The stitch drawing explains fixation, not every needle pass of a named suture.
Avoid displacement, folding or excessive compression. Blue denotes suture, not an incision.
10. Advance the flap coronally (2:02)
The released flap moves toward the tooth crown and covers the graft as it settles onto the recipient. The simplified motion illustrates the relationship between the graft and covering flap. This is an immediate surgical position.
This example uses full graft coverage; exposure and final margin position are not interchangeable across techniques.
11. Support and close (2:20)
The modified sling passes buccal to palatal through the mesial surgical and anatomical papillae, around the palatal cingulum, back beneath the distal contact, then through the distal papillae and beneath the mesial contact to a buccal knot. The return beneath a contact is distinct from the tissue bite. An incisal inset reveals the concealed palatal route. Separate interrupted stitches adapt the releases. This is a teaching synthesis of the cited descriptions, with simplified instrument handling.
Stabilize a passively positioned flap; do not use tight sutures to overcome residual tension.
12. Review both sites over time (2:54)
Give individual postoperative instructions, wound-protection advice and review arrangements. Healing, suture removal and later root-coverage assessment occur after this sequence. No long-term outcome is predicted by the final illustration.
Published technique studies do not make this film a complete operative manual or replace supervised training.
Anatomical model credits
Tooth models: University of Dundee, School of Dentistry, licensed under Creative Commons Attribution 4.0. Adapted for this teaching animation: scaled, mirrored, arranged and retextured. Surrounding tissues, grafts and procedural animation are original teaching models. These adaptations do not imply Dundee endorsement or clinical validation.
Original models: Central incisorLateral incisorCanineFirst premolarSecond premolarFirst molarSecond molar
Read the full model and scientific credits04 / Tunnel-based access
Preserve the bridge of tissue.
A tunnel connects recipient spaces beneath the marginal tissues while preserving papillary continuity. The graft is introduced beneath this envelope. Tunnel is a family of techniques: preparation depth, papillary mobilization and coronal advancement differ between protocols.[6][10][13][14]
Concept 1
Connect the recipient space
Sulcular access leads to an underlying corridor. Maintain papillary continuity and avoid perforating a thin envelope.
- Sulcular access: distinct from a vertical release.
- Connected concealed corridor beneath the tissues.
Concept 2
Adapt the graft
Introduce and adapt the CTG beneath the envelope. The graft should contact its recipient bed without folds or displacement.
- Buried CTG; transparency reveals its position.
- Papillary bridge retained above the corridor.
Concept 3
Support the envelope
Select a fixation and suspension approach suited to the graft and tissue. Coronal advancement is part of some, rather than all, tunnel protocols.
- Contact-supported suspension: force concept only.
- Coronal tissue support without excessive tension.
- When to consider it
- Preserved papillary continuity and a connected recipient envelope fit the defect, the tissue thickness and the operator’s training.
- What to question
- Restricted visibility, thin-tissue perforation and graft handling can make this demanding. “Minimally invasive” does not guarantee less postoperative pain.
- How to read the evidence
- A CAF-versus-tunnel RCT using CTG in both groups found similar root coverage, with more early morbidity in its tunnel group. The finding concerns those tested protocols, not every tunnel variation.
Conceptual sequence, not a complete operative protocol. Planes, grafts and stabilization differ across published variants.[6][10][13][14]
05 / Vestibular incision subperiosteal tunnel access
Reach the site from a remote incision.
The original VISTA concept uses a remote vestibular incision and subperiosteal access, keeping marginal incisions away from the recession sites. Coronal anchorage helps maintain the intended margin position. Later CTG-based and modified VISTA protocols are not identical to the original report.[7][8]
Concept 1
Enter remotely
The original maxillary-anterior description accesses the tissues through a midline vestibular incision. The concealed preparation extends toward the affected margins.
- Remote vestibular access in the midline.
- Subperiosteal corridor; its boundary is not a surface cut.
Concept 2
Place the selected material
A CTG is shown here to explain a graft-based modification. The original 2011 report instead used rhPDGF-BB with a β-TCP scaffold.
- CTG in a modified graft-based concept.
- The overlying tissue is transparent, not absent.
Concept 3
Anchor and close
Coronally anchored sutures support the margins from fixation points on the facial crowns. Close the remote access separately.
- Facial crown anchor points, shown in pale green.
- Sutures maintain the planned tissue position.
- When to consider it
- Remote access and a subperiosteal preparation are appropriate to the site and the particular protocol being taught.
- What to question
- Avoid treating VISTA as a synonym for every tunnel. Vestibular anatomy, access, tissue mobility and reliable anchorage still require assessment.
- How to read the evidence
- The original publication is a technique report with two cases. A later m-VISTA-versus-CAF RCT in multiple RT2 defects found no significant difference in mean coverage at 6 or 12 months.
Conceptual sequence, not a complete operative protocol. Planes, grafts and stabilization differ across published variants.[7][8]
Know which layer you are mobilizing.
Subperiosteal means beneath the periosteum. A partial-thickness preparation leaves tissue over the underlying surface. These sections are away from a root, where bone is present.
- One partial-thickness example: access within the connective tissue.
- Subperiosteal concept: periosteum stays with the elevated tissues.
Tunnel modifications can combine planes. The original VISTA preparation and the partial-thickness tunnel example described by Ronco are not the same dissection. The dashed lines mark a plane, not a gap left open at closure.[7][12]
06 / Suture atlas
Choose the stitch for the job.
Separate three jobs: adapting wound edges, immobilizing a graft and supporting coronal tissue position. A combination may be needed. More traction is not a substitute for adequate tissue mobilization.[9][10][12]
Simple interrupted
Independent adaptation of a local wound edge or a separate access incision.
- External loop; knot offset from the wound.
- Concealed return through tissue.
Clinical attention: A closure stitch alone does not create passive coronal advancement. Keep the knot away from the incision line.[9]
Horizontal mattress
Spreads support over a wider surface through two adjacent passes; may help graft adaptation.
- Two adjacent concealed cross-wound passes.
- External turns on either side.
Clinical attention: Excessive compression can compromise perfusion. This is a surface view, not the vertical mattress cross-section beside it.[9][12]
Vertical mattress
A deeper far–far and a shallower near–near component control tissue adaptation.
- Far–far component shown deeper.
- Near–near component shown superficially.
Clinical attention: Bite depth and position depend on the tissue and objective. The diagram prescribes no fixed clinical distances.[9][12]
Tooth-supported sling
Uses a tooth as an anchor to support the marginal tissues; continuous designs can also secure a tunnel graft.
- External tooth-supported loop.
- Concealed lingual segment shown dashed.
Clinical attention: An independent sling and Allen’s subpapillary continuous sling are related concepts, not interchangeable step-by-step protocols.[9][10]
Double-crossed suspension
Illustrates coronal support and close adaptation of the gingivopapillary complex in the double-crossed family.
- Supported contact-point anchorage.
- Two crossing support lines: force concept.
Clinical attention: The drawing shows the mechanical objective, not every needle pass of Zuhr’s named technique. Thin papillae require particular care.[11][12]
Coronally anchored suture
Supports coronal positioning from facial crown fixation points, as described in VISTA.
- Facial composite fixation points.
- Coronal support of the marginal tissues.
Clinical attention: The anchor must be reliable, while the tissue remains passively positionable. This is different from contact-point suspension.[7]
Stability with perfusion.
Check passive adaptation, graft contact and absence of folding or movement. Match the needle, material, diameter and support pattern to tissue thickness and the task. Avoid strangulation and unnecessary tissue trauma. Timing of removal and postoperative care belong to the individual protocol.[9][12]
The atlas explains mechanics. In particular, the double-crossed and anchored drawings are conceptual: use the original articles and supervised practical teaching to learn the complete needle route and knot sequence.
07 / Apply the chapter
Explain the choice before naming the technique.
Teaching scenario: two adjacent RT1 recessions, a thin phenotype and preserved papillae. The patient is concerned about appearance. This description is incomplete by design: the operation cannot be selected from these facts alone.
What else would you record?
Plaque control and periodontal stability, recession depth and width, root-surface condition, CEJ, keratinized tissue, vestibular / frenum anatomy, tooth position and patient preferences. Confirm the attachment measurements.
What would justify a graft?
Explain the intended gain: coverage, thickness or keratinized tissue. Discuss recipient biology, donor-site morbidity and alternatives. A thin phenotype contributes to the decision; a technique name does not determine the material.
How would you test your closure plan?
Ask whether the tissue reaches the planned position passively, what fixes the graft, what supplies anchorage, and whether the support preserves perfusion. Then identify the exact published variant and its limits.
Read comparisons fairly.
A comparison of CAF + CTG with tunnel + CTG differs from a comparison that also changes the biomaterial. Check defect type, graft, operator experience, follow-up, complete versus mean coverage and patient-reported outcomes. A technical report demonstrates an approach; it does not establish superiority.[5][6][8]
08 / Scientific reading
Start with the original descriptions.
Selected foundational publications, comparative research and scholarly reviews. Short descriptive titles below identify each paper’s role. This is a focused teaching bibliography, not an exhaustive systematic review. Figures are original OsteoPerio schematics; no article figures have been reproduced.
Read the original descriptions alongside Tavelli and colleagues’ 2026 review of how CAF and tunnel approaches evolved. A current synthesis helps distinguish established concepts from later modifications.[13]
[1] Classification study / 2011
Interproximal attachment, recession classification and root-coverage predictionCairo F, Nieri M, Cincinelli S, et al. Journal of Clinical Periodontology. 38:661–666.
Defines RT1, RT2 and RT3 using interproximal versus buccal attachment loss.
[2] World Workshop narrative review / 2018
Mucogingival conditions in the natural dentitionCortellini P, Bissada NF. Journal of Periodontology. 89(Suppl 1):S204–S213.
Assessment includes phenotype, root-surface condition, symptoms, patient concerns and monitoring.
[3] Critical review / 2014
Soft-tissue replacement grafts: critical elements in design and executionZuhr O, Bäumer D, Hürzeler M. Journal of Clinical Periodontology. 41(Suppl 15):S123–S142.
Donor tissues, substitutes, harvesting considerations and recipient-site biology.
[4] Clinical case series / 2000
Treatment of multiple recession-type defects in patients with esthetic demandsZucchelli G, De Sanctis M. Journal of Periodontology. 71:1506–1514.
A multiple-recession CAF design; not a guarantee for every defect or every CAF variant.
[5] Systematic review / 2014
Efficacy of periodontal plastic surgery for localized gingival recessionsCairo F, Nieri M, Pagliaro U. Journal of Clinical Periodontology. 41(Suppl 15):S44–S62.
Supports CAF with CTG for selected single recessions; findings depend on the comparator and defect.
[6] Randomized clinical trial / 2016
CAF + CTG versus tunnel + CTG: morbidity and root-coverage outcomesGobbato L, Nart J, Bressan E, et al. Clinical Oral Investigations. 20:2191–2202.
Similar coverage in this trial; the tunnel group reported more early morbidity. Less invasive terminology does not ensure less pain.
[7] Technique report / two clinical cases / 2011
VISTA access and coronally anchored suturing: the original reportZadeh HH. International Journal of Periodontics & Restorative Dentistry. 31:653–660.
Remote vestibular incision, subperiosteal tunnel and coronal anchorage. The original report used rhPDGF-BB with a β-TCP scaffold.
[8] Randomized clinical trial / 2023
Modified VISTA versus CAF with CTG for multiple RT2 recessionsFernández-Jiménez A, Estefanía-Fresco R, García-De-La-Fuente AM, et al. Clinical Oral Investigations. 27:505–517.
No statistically significant between-group difference in mean coverage at 6 or 12 months; modified VISTA is not identical to the original protocol.
[9] Technical review / 1996
Suturing techniques for periodontal plastic surgeryMoore RL, Hill M. Periodontology 2000. 11:103–111.
Conventional suture patterns, wound adaptation and tissue stabilization.
[10] Technical report / 2010
Subpapillary continuous sling suturing for soft-tissue grafting with tunnelingAllen EP. International Journal of Periodontics & Restorative Dentistry. 30:479–485.
Combines graft fixation and advancement of the pouch margins in a continuous sling design.
[11] Technical report / 2009
The double-crossed suture for periodontal and implant plastic surgeryZuhr O, Rebele SF, Thalmair T, et al. European Journal of Esthetic Dentistry. 4:338–347.
A named suspensory suture design. Consult the original description for the complete needle route.
[12] Technical case report / 2016
A suturing approach for tissue displacement in minimally invasive periodontal plastic surgeryRonco V, Dard M. Clinical Case Reports. 4:831–837.
Anchored horizontal and vertical mattress concepts; stabilization and compression with attention to tissue trauma.
[13] Contemporary narrative review / 2026
Root-coverage surgery: the evolution of CAF and tunnel approachesTavelli L, Yilmaz B, Steiner Back L, et al. Journal of Periodontology. 97:983–1009.
Places access designs and their modifications in context, including different preparation planes and coronal positioning concepts.
[14] Microsurgical tunnel case report / 2007
Gingival recession coverage with a modified microsurgical tunnelZuhr O, Fickl S, Wachtel H, et al. International Journal of Periodontics & Restorative Dentistry. 27:457–463.
Describes a split-flap modification with microsurgical instruments. A technical report does not establish universal superiority.
[15] Randomized controlled trial / 2010
Patient morbidity and root coverage after subepithelial and de-epithelialized graftsZucchelli G, Mele M, Stefanini M, et al. Journal of Clinical Periodontology. 37:728–738.
Compares CAF with grafts obtained through trap-door harvesting or extraoral de-epithelialization of an epithelialized graft. Donor techniques and wound management differ.
[16] Clinical follow-up study / 2007
Modified CAF for isolated recession-type defects: three-year resultsde Sanctis M, Zucchelli G. Journal of Clinical Periodontology. 34:262–268.
Isolated-recession flap design with differentiated preparation thickness. Distinct from the multiple-recession envelope example.
[17] Randomized controlled clinical trial / 2022
De-epithelialized graft versus single-incision subepithelial graft with CAFMashaly M, Ghallab NA, Elbattawy W, Ezz Elarab A. Contemporary Clinical Dentistry. 13:227–235.
Describes isolated CAF preparation, extraoral epithelial removal, donor-wound protection, graft fixation and covering-flap stabilization. Study dimensions are not universal prescriptions.
[18] Experimental human-cadaver study / 2024
Histological assessment of a novel de-epithelialization method for connective tissue grafts harvested from the palateBara-Gaseni N, Jorba-Garcia A, Alberdi-Navarro J, et al. Clinical Oral Investigations. 28:343.
Documents a 2 mm marginal collar, horizontal and connecting incisions, split-thickness harvesting and extraoral scalpel de-epithelialization. These are study-specific methods, not universal safe dimensions.
[19] Narrative review / 2026
Mucogingival surgery techniques: Coronally advanced flap versus tunnel approachAroca S, Di Domenico GL, de Sanctis M. Journal of Periodontology. 97:1714–1732.
Details mesial and distal papillary passes, palatal cingulum anchorage and returns beneath the contacts for a modified sling. Its clinical figure illustrates multiple CAF; it is not an isolated-recession DGG trial.